Part II: Needleman-Wunsch Algorithm

Instructions:

1. Go to

https://www.ebi.ac.uk/Tools/sss/ and select GGSEARCH then protein. This will do

a Needleman-Wunsch global alignment on your protein sequence.

2. Choose UniProtKB/TrEMBL as your database (step 1)

3. Paste in your STS protein query sequence (step 2)

4. Click More options... on step 3 and choose BLOSUM50 as your scoring matrix and then

click Submit.

Questions:

1. What is the name and score of your top hit?

2. How do these results differ from what you got with the Smith-Waterman algorithm

(SSEARCH)?

3. Why do you think the results differed?