Bonus: Determine the primary sequence of the following peptide. (10 Pts)

A. Trypsin treatment generated the following fragments:

(LFVCYMGFR) (HDITNAATYR)

(AEK)

(CPS) (K) (QIVAAR) (LWVANK)

B. Chymotrypsin treatment generated the following fragments:

(AEKL) (F) (VCY) (MGF)

(RHDITNAATY)

(RL)

(W) (VANKKQIVAARCPS)

C. Thermolysin treatment generated the following fragments:

(LF) (VCY) (MGFRHD) (ITNAATYR) (AEK)

(LW) (VANKKQ) (1) (V) (AARCPS)

Legend:

Trypsin = Lys & Arginine (R.)

Chymotrypsin = Tyr, Trp, Phe, Leu (R.)

Thermolysin = Leu, Val, lle, Met (R₂)