BMOL20070 Assignment 3 (Practicals 4-8-9)

You must answer all questions typed and submit as a .pdf on Brightspace. The deadline for

Assignment 3 is

April. The Assignment component for

BMOL20070 is woru 35%0. Al 5 assignments are equally weighted. Remember, late

submission will incur in loss of marks.

Practical 4: Rat Ileum

Question 1.

Carbachol causes contractions the isolated rat ileum to contract. The responses obtained

to increasing concentrations of carbachol are tabulated below. Graph the data as log

concentration versus % maximum response, using Excel or another spreadsheet

program. Use the graph to estimate the EC50 value for carbachol and indicate its position

on the graph. Include the graph in your submitted file.

Conc (M)

1.00E-09

3.00E-09

1.00E-08

3.00E-08

1.00E-07

3.00E-07

1.00E-06

3.00E-06

Exp 1

(% max response)

0

10

45

57

74

95

100

100

Exp 2

(% max response)

0

9

35

69

81

85

100

98

Exp 3

(% max response)

0

20

48

72

91

96

99

99

Question 2.

Briefly outline an experiment that would determine the relative potencies of two agonists

acting at different receptors e.g. carbachol and serotonin.